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( A ) Preparation procedure and antithrombus mechanism of SiH@UK/Fib. ( B ) TEM result and element mapping of SiH NS. ( C ) AFM images of SiH and SiH@UK/Fib. ( D ) Thickness distribution of the SiH and SiH@UK/Fib quantified from (C). ( E ) Hydrodynamic diameter distributions of SiH@Fib after treated with thrombin for different time periods. ( F ) SEM results of the thrombus in 10-min incubation with SiH@Fib in PBS and plasma, respectively. ( G ) Confocal image of tetramethyl rhodamine isothiocynate (TRITC)–labeled thrombus (red) in 10-min incubation with fluorescein isothiocyanate <t>(FITC)–labeled</t> SiH@Fib.
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( A ) Preparation procedure and antithrombus mechanism of SiH@UK/Fib. ( B ) TEM result and element mapping of SiH NS. ( C ) AFM images of SiH and SiH@UK/Fib. ( D ) Thickness distribution of the SiH and SiH@UK/Fib quantified from (C). ( E ) Hydrodynamic diameter distributions of SiH@Fib after treated with thrombin for different time periods. ( F ) SEM results of the thrombus in 10-min incubation with SiH@Fib in PBS and plasma, respectively. ( G ) Confocal image of tetramethyl rhodamine isothiocynate (TRITC)–labeled thrombus (red) in 10-min incubation with fluorescein isothiocyanate <t>(FITC)–labeled</t> SiH@Fib.
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( A ) Preparation procedure and antithrombus mechanism of SiH@UK/Fib. ( B ) TEM result and element mapping of SiH NS. ( C ) AFM images of SiH and SiH@UK/Fib. ( D ) Thickness distribution of the SiH and SiH@UK/Fib quantified from (C). ( E ) Hydrodynamic diameter distributions of SiH@Fib after treated with thrombin for different time periods. ( F ) SEM results of the thrombus in 10-min incubation with SiH@Fib in PBS and plasma, respectively. ( G ) Confocal image of tetramethyl rhodamine isothiocynate (TRITC)–labeled thrombus (red) in 10-min incubation with fluorescein isothiocyanate <t>(FITC)–labeled</t> SiH@Fib.
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( A ) Preparation procedure and antithrombus mechanism of SiH@UK/Fib. ( B ) TEM result and element mapping of SiH NS. ( C ) AFM images of SiH and SiH@UK/Fib. ( D ) Thickness distribution of the SiH and SiH@UK/Fib quantified from (C). ( E ) Hydrodynamic diameter distributions of SiH@Fib after treated with thrombin for different time periods. ( F ) SEM results of the thrombus in 10-min incubation with SiH@Fib in PBS and plasma, respectively. ( G ) Confocal image of tetramethyl rhodamine isothiocynate (TRITC)–labeled thrombus (red) in 10-min incubation with fluorescein isothiocyanate <t>(FITC)–labeled</t> SiH@Fib.
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( A ) Preparation procedure and antithrombus mechanism of SiH@UK/Fib. ( B ) TEM result and element mapping of SiH NS. ( C ) AFM images of SiH and SiH@UK/Fib. ( D ) Thickness distribution of the SiH and SiH@UK/Fib quantified from (C). ( E ) Hydrodynamic diameter distributions of SiH@Fib after treated with thrombin for different time periods. ( F ) SEM results of the thrombus in 10-min incubation with SiH@Fib in PBS and plasma, respectively. ( G ) Confocal image of tetramethyl rhodamine isothiocynate (TRITC)–labeled thrombus (red) in 10-min incubation with fluorescein isothiocyanate <t>(FITC)–labeled</t> SiH@Fib.
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( A ) Preparation procedure and antithrombus mechanism of SiH@UK/Fib. ( B ) TEM result and element mapping of SiH NS. ( C ) AFM images of SiH and SiH@UK/Fib. ( D ) Thickness distribution of the SiH and SiH@UK/Fib quantified from (C). ( E ) Hydrodynamic diameter distributions of SiH@Fib after treated with thrombin for different time periods. ( F ) SEM results of the thrombus in 10-min incubation with SiH@Fib in PBS and plasma, respectively. ( G ) Confocal image of tetramethyl rhodamine isothiocynate (TRITC)–labeled thrombus (red) in 10-min incubation with fluorescein isothiocyanate <t>(FITC)–labeled</t> SiH@Fib.
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( A ) Preparation procedure and antithrombus mechanism of SiH@UK/Fib. ( B ) TEM result and element mapping of SiH NS. ( C ) AFM images of SiH and SiH@UK/Fib. ( D ) Thickness distribution of the SiH and SiH@UK/Fib quantified from (C). ( E ) Hydrodynamic diameter distributions of SiH@Fib after treated with thrombin for different time periods. ( F ) SEM results of the thrombus in 10-min incubation with SiH@Fib in PBS and plasma, respectively. ( G ) Confocal image of tetramethyl rhodamine isothiocynate (TRITC)–labeled thrombus (red) in 10-min incubation with fluorescein isothiocyanate <t>(FITC)–labeled</t> SiH@Fib.
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( A ) Preparation procedure and antithrombus mechanism of SiH@UK/Fib. ( B ) TEM result and element mapping of SiH NS. ( C ) AFM images of SiH and SiH@UK/Fib. ( D ) Thickness distribution of the SiH and SiH@UK/Fib quantified from (C). ( E ) Hydrodynamic diameter distributions of SiH@Fib after treated with thrombin for different time periods. ( F ) SEM results of the thrombus in 10-min incubation with SiH@Fib in PBS and plasma, respectively. ( G ) Confocal image of tetramethyl rhodamine isothiocynate (TRITC)–labeled thrombus (red) in 10-min incubation with fluorescein isothiocyanate (FITC)–labeled SiH@Fib.

Journal: Science Advances

Article Title: In situ coagulation environment regulation–assisted thrombus clearance via hydrogenated silicon-based nanothrombolytics

doi: 10.1126/sciadv.aea4782

Figure Lengend Snippet: ( A ) Preparation procedure and antithrombus mechanism of SiH@UK/Fib. ( B ) TEM result and element mapping of SiH NS. ( C ) AFM images of SiH and SiH@UK/Fib. ( D ) Thickness distribution of the SiH and SiH@UK/Fib quantified from (C). ( E ) Hydrodynamic diameter distributions of SiH@Fib after treated with thrombin for different time periods. ( F ) SEM results of the thrombus in 10-min incubation with SiH@Fib in PBS and plasma, respectively. ( G ) Confocal image of tetramethyl rhodamine isothiocynate (TRITC)–labeled thrombus (red) in 10-min incubation with fluorescein isothiocyanate (FITC)–labeled SiH@Fib.

Article Snippet: Rabbit anti-Nrf2 antibody, rabbit anti–p-Nrf2 antibody, rabbit anti-CD41 antibody, rabbit anti–PAI-1 antibody, rabbit anti–collagen I antibody, FITC-labeled goat anti-rabbit immunoglobulin G (IgG) antibody, and Cy3-labeled goat anti-rabbit IgG antibody were obtained from Bioss Biotechnology Co. Ltd. (Beijing, China).

Techniques: Incubation, Clinical Proteomics, Labeling